A process for in vitro differentiation of neuronal stem...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0797 (2010.01) C12N 5/079 (2010.01) A61K 31/352 (2006.01) A61K 35/30 (2006.01) A61K 38/17 (2006.01) A61K 39/395 (2006.01) C12N 15/09 (2006.01) C12Q 1/02 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2551259

The method for in vitro differentiation of neuronal stem cells comprises the following: the cells are brought into contact with a substance which inhibits a reaction of the Wnt signal transduction path, and said cells are cultivated in conditions enabling the cells to multiply and/or differentiate. In a preferrred embodiment of the method, the neuronal stem cells differentiate to form cells which are similar to brain cells.

L'invention concerne un procédé de différentiation in vitro de cellules souches neuronales consistant à mettre les cellules en contact avec une substance inhibant une réaction de la voie de transduction de signal Wnt, et à cultiver les cellules dans des conditions permettant une multiplication et/ou une différentiation des cellules. Dans un mode de réalisation préféré, les cellules souches neuronales se différencient de manière à former des cellules semblables à des cellules cérébrales.

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