C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/415 (2006.01) C07K 1/14 (2006.01) C07K 19/00 (2006.01) C12N 7/02 (2006.01) C12N 15/09 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2322616
The present invention features a method for isolating and purifying viruses, proteins and peptides of interest from a plant host which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating viruses, proteins and peptides of interest than those methods described in the prior art. In general, the present method of isolating viruses, proteins and peptides of interest comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.
Cette invention, qui a trait à une technique applicable à grande échelle permettant d'isoler et de purifier des virus des protéines et des peptides provenant d'une plante hôte, porte, de surcroît, sur une technique plus efficace pour ce faire que les procédés existants à ce jour. Cette technique consiste, généralement, à homogénéiser une plante pour produire un jus vert, à ajuster le pH de ce jus et à le chauffer, à séparer l'espèce cible, qu'il s'agisse du virus ou de la protéine et/ou du peptide, des autres constituants et ce, par un ou plusieurs cycles de centrifugation, de remise en suspension et d'ultrafiltration et enfin, à purifier les particules virales par précipitation par polyéthylène glycol ou à purifier les protéines et les peptides par chromatographie et/ou précipitation de sel.
Garger Stephen J.
Holtz R. Barry
Mcculloch Michael J.
Turpen Thomas H.
Biosource Technologies Inc.
Kentucky Bioprocessing Llc
Smart & Biggar
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