A process for isolation and purification of a target protein...

Details A process for isolation and purification of a target protein... A process for isolation and purification of a target protein...

A - Human Necessities

61

L

A61L 2/00 (2006.01) C07K 1/16 (2006.01) C07K 14/47 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2696865

A process for isolation and purification of a target protein by chromatography wherein the chromatography removes or depletes prions (PrP sc), comprising the steps of contacting a potentially PrP sc contaminated sample comprising a target protein with a multimodal chromatographic material; setting buffer conditions so that the target protein is bound to the multimodal chro-matographic material and whereas PrP sc is not binding to the multimodal chromatographic material; followed by elution of the target protein.a process for isolation and purification of a target protein free of prion protein (prp sc).

L'invention porte sur un procédé pour l'isolement et la purification d'une protéine cible par chromatographie, la chromatographie éliminant ou appauvrissant les prions (PRPSC). Le procédé consiste à mettre en contact un échantillon potentiellement contaminé par le PRPSC comportant une protéine cible avec un matériau chromatographique multimodal; à établir des conditions de tampon de telle sorte que la protéine cible est liée au matériau chromatographique multimodal alors que le PRPSC n'est pas lié au matériau chromatographique multimodal; à faire suivre par l'élution de la protéine cible. L'invention porte également sur un procédé pour l'isolement et la purification d'une protéine cible exempte de la protéine de prion (PRPSC).

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