A process for obtaining transgenic leguminous plants...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01)

Patent

CA 2305576

The present invention refers to a process for producing transgenic leguminous plants containing exogenous DNA, which comprises the steps of introducing exogenous genes into cells of the apical meristem of embryonic axis of leguminous plants by the biobalistic method; inducing multiple shooting of the cells in the apical meristematic region modified in the preceding step by cultivating their embryonic axis in a medium containing a multiple shooting inducer; and selecting the meristematic cells of the apical region, transformed by further cultivation of said embryonic axis in a medium containing a molecule which concentrates in the apical meristematic region of said leguminous plants embryos.

L'invention concerne un procédé pour produire des légumineuses transgéniques contenant de l'ADN exogène, consistant à introduire des gènes exogènes dans des cellules du méristème apical de l'axe embryonnaire de légumineuses par la méthode biobalistique, à induire la formation de multiples pousses des cellules dans la région méristématique apicale modifiée lors de l'étape précédente, par la mise en culture de leur axe embryonnaire dans un milieu contenant un inducteur de formation de pousses multiples, et à sélectionner les cellules méristématiques de la région apicale, transformées par nouvelle mise en culture dudit axe embryonnaire dans un milieu contenant une molécule se concentrant dans la région méristématique apicale desdits embryons de légumineuses.

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