A process for preparation of timosaponin b ii

Details A process for preparation of timosaponin b ii A process for preparation of timosaponin b ii

C - Chemistry, Metallurgy

07

J

C07J 71/00 (2006.01)

Patent

CA 2564889

A precess for preparation of timosaponin B II, which uses Chinese traditional drug Anemarrhena Rhizome or newly collected rhizoma or fibrous root of Anemarrhena Asphodeloides Bge. as raw material, and comprises isolation of tiBosaponin B II by one or more steps selected from solvent extraction, resin adsorption, polyamide chromatography, inversed phase column chromatography, column chromatography on Sephadex LH-20 and so on in combination with conventional drying method such as vacuum drying, freeze drying, spray drying and so on. Timosaponin B II obtained by the present process is of over 90% purity, and the process is simple and applicable and suitable for industrial production.

L'invention concerne un procédé de préparation de timosaponine B II, consistant à utiliser en tant que matière première le rhizome d'Anemarrhena qui est un médicament chinois traditionnel, ou les rhizomes ou racines fibreuses fraîchement récoltés de Anemarrhena Aspholdeloides Bge, et consiste à isoler la timosaponine B II en une ou plusieurs étapes choisies parmi les suivantes: extraction par solvant, adsorption sur résine, chromatographie sur polyamide, chromatographie sur colonne en phase inverse, chromatographie sur colonne sur Sephadex LH-20 etc., combinées avec un processus de séchage conventionnel tel que séchage sous vide, lyophilisation, séchage par vaporisation etc. La timosaponine B II obtenue par ce procédé présente une pureté supérieure à 90%. Le procédé décrit est simple, et il est applicable et adapté à la production industrielle.

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