A process for preparing nicotinamide adenine dinucleotide...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/36 (2006.01) C12N 9/12 (2006.01)

Patent

CA 2452399

A novel process for producing nicotinamide adenine dinucleotide phosphate (NADP) which comprises using polyphosphoric acid or its salt and nicotinamide adenine dinucleotide (NAD+) as a substrate and phosphorylating the substrate with the use of a polyphosphoric acid-depending NAD+ kinase originating in a mycobacterium, characterized in that the liquid reaction mixture contains fro 0.1 to 15% by weight of polyphosphoric acid or its salt and from 5 to 150 mM of a divalent metal ion.

La présente invention concerne un nouveau procédé de production de nicotinamide-adénine-dinucléotide-phosphate (NADP) comportant l'utilisation d'un acide polyphosphorique ou son sel et du nicotinamide-adénine-dinucléotide (NAD?+¿) en tant que substrat et la phosphorylation du substrat à l'aide d'une kinase NAD?+¿ dépendant de l'acide polyphosphorique d'origine mycobactérienne. Le procédé est caractérisé en ce que mélange réactionnel liquide contient de 0,1 à 15 % en poids d'acide polyphosphorique ou de son sel et de 5 à 150 nM d'un ion métallique divalent.

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