A process for producing a recombinant polypeptide involving...

C - Chemistry – Metallurgy – 12 – P

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12P 21/02 (2006.01) C07K 14/755 (2006.01) C12N 9/99 (2006.01)

Patent

CA 2253287

The present invention relates to a process for reducing the detrimental influence of certain proteases on recombinant human protein and polypeptide molecules, by adding an inhibitor of metal-dependent proteases or chymotrypsins to the cell culture medium. The invention also relates to a cell culture medium for cultivating cells expressing and secreting a biologically active recombinant human polypeptide containing an inhibitor of metal-dependent proteases or chymotrypsins, or a combination thereof. The invention further relates to use of recombinant factor VIII which has been produced in a cell culture medium according to the present process for the manufacture of a medicament for administration to a patient having the symptoms of hemophilia A. Also, the invention relates to a method for treatment of hemophilia A by administration of a therapeutically effective amount of recombinant factor VIII which has been produced in a cell culture medium according to the present process.

Procédé pour réduire l'influence nocive de certaines protéases sur les molécules de protéines et de polypeptides humains recombinés, par l'adjonction d'un inhibiteur de protéases métal-dépendantes ou de chymotrypsines au milieu de culture cellulaire. L'invention concerne également un milieu de culture cellulaire pour cultiver des cellules exprimant et sécrétant un polypeptide humain recombiné, biologiquement actif et contenant un inhibiteur de protéases métal-dépendantes ou de chymotrypsines, ou une combinaison des deux. L'invention porte en outre sur l'utilisation du facteur recombiné VIII produit dans un milieu de culture conforme au procédé décrit, pour la fabrication d'un médicament pouvant être administré à un patient présentant les symptômes de l'hémophilie A. Une méthode de traitement de l'hémophilie A comprenant l'administration d'une quantité thérapeutiquement active de facteur recombiné VIII produit dans un milieu de culture conforme au procédé décrit est également proposée.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

A process for producing a recombinant polypeptide involving... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with A process for producing a recombinant polypeptide involving..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and A process for producing a recombinant polypeptide involving... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1712940

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.