A process for production and subsequent ex vitro sowing and...

A - Human Necessities – 01 – H

Patent

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A01H 4/00 (2006.01)

Patent

CA 2341455

A process of producing somatic seedlings from a somatic embryo. The process comprising pre-germinating somatic embryos, placing the pre-germinated somatic embryos into a state of physiological dormancy, sowing the pre-germinated physiologically dormant somatic embryos onto or into germination media, and propagating the sown pre-germinated somatic embryos in environmental conditions manipulated to facilitate imbibition, germination, and development into complete seedlings possessing shoots and roots. Advantageously, the process may be carried out with "naked" embryos (i.e., non-encapsulated or otherwise coated embryos) using conventional seed handling equipment, growing mixes, and plant propagation environments. The process does not require the use of aseptic techniques or sterilized media or equipment. The invention also relates to pre-germinated embryos and seedlings produced by the process.

L'invention concerne un procédé de production de semis somatiques provenant d'un embryon somatique. Le procédé consiste: à faire pré-germer des embryons somatiques; à placer les embryons somatiques pré-germés à l'état de dormance physiologique; à ensemencer les embryons somatiques pré-germés et physiologiquement en dormance sur un support de germination ou à l'intérieur de ce support; puis à propager les embryons somatiques pré-germés ensemencés dans des conditions ambiantes manipulées pour en faciliter l'imbibition, la germination et le développement en semis complets présentant des pousses et des racines. On peut réaliser le procédé avec avantage en utilisant des embryons "nus" (c.-à-d. non encapsulés ou différemment enrobés) et du matériel de manipulation de semis classique, des mélanges de croissance et des milieux de propagation de végétaux. Le procédé ne nécessite pas le recours à des techniques aseptiques ou à des milieux ou du matériel stérilisé. L'invention concerne également des embryons et des semis pré-germés produits selon le procédé de l'invention.

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