A process for purifying apolipoprotein a or apolipoprotein e...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/775 (2006.01) C07K 1/18 (2006.01) C07K 1/36 (2006.01) C07K 14/755 (2006.01)

Patent

CA 2274241

The present invention relates to a process for purifying apolipoprotein A (ApoA) or apolipoprotein E (ApoE) from human plasma, by obtaining a fraction of human plasma containing said ApoA or ApoE, prepurifying said fraction in at least one step, binding said ApoA or ApoE to an anion-exchange chromatography gel, and thereafter eluting said ApoA or ApoE from said anion-exchange chromatography gel. The thus produced ApoA or ApoE can be used for the manufacture of a medicament in the treatment of atherosclerosis and cardiovascular diseases, or peripheral atherosclerosis and sepsis as well as in a method for treatment of atherosclerosis and cardiovascular diseases, or peripheral atherosclerosis and sepsis when administered in a therapeutically effective amount.

Cette invention a trait à une technique de purification d'alipoprotéine A (ApoA) ou d'alipoprotéine E (ApoE) provenant de plasma sanguin humain, laquelle technique consiste à obtenir une fraction de plasma humain contenant l'ApoA ou l'ApoE, à procéder à une première purification de cette fraction, en une étape au moins, à lier ladite ApoA ou ApoE à un gel chromatographique échangeur d'anions et, enfin, à éluer ladite ApoA ou ApoE de ce gel chromatographique échangeur d'anions. On peut utiliser l'ApoA ou l'ApoE ainsi obtenue à l'élaboration d'un médicament visant à traiter l'athérosclérose et des affections d'ordre cardio-vasculaire ou à traiter l'artériosclérose périphérique et des maladies infectieuses. On peut également les utiliser dans le cadre d'une méthode de traitement de l'athérosclérose et d'affections d'ordre cardio-vasculaire ou de traitement de l'artériosclérose périphérique et de maladies infectieuses et ce, en les administrant sous des quantités efficaces du point de vue thérapeutique.

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