A process for recovering polypeptides that unfold reversibly...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/13 (2006.01) C07K 16/18 (2006.01) C12N 15/09 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2525120

The invention relates to polypeptides that unfold reversibly (e.g., unfolds when heated and refolds when cooled), to repertoires containing polypeptides that unfold reversibly and to libraries that contain polypeptides that unfold reversibly or nucleic acids that encode polypeptides that unfold reversibly. The invention further relates to processes for producing a library enriched in polypeptides that unfold reversibly or nucleic acids encoding polypeptides that unfold reversibly, processes for selecting and/or isolating polypeptides that unfold reversibly, and to methods for producing a polypeptide that unfolds reversibly.

La présente invention concerne des polypeptides qui se déplient de façon réversible, par exemple, se déplient lorsqu'ils sont chauffés et se replient en refroidissant. L'invention concerne également des répertoires contenant des polypeptides qui se déplient de façon réversible, ainsi que des échantillothèques contenant des polypeptides qui se déplient de façon réversible, ou des acides nucléiques qui codent des polypeptides qui se déplient de façon réversible. L'invention concerne aussi des traitements permettant de produire une échantillothèque enrichie en polypeptides qui se déplient de façon réversible ou des acides nucléiques qui codent des polypeptides qui se déplient de façon réversible. L'invention concerne enfin, d'une part des procédés permettant de sélectionner et/ou d'isoler des polypeptides qui se déplient de façon réversible, et d'autre part des procédés pour produire un polypeptide qui se déplie de façon réversible.

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