A process for the production of transgenic plants having a...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2735950

A process for the production of transgenic plants that have a high starch content and yield and a high amylose / amylopectin ratio. The alpha-1 ,4-glucan phosphorylases (GPs) catalyze the reversible cutting of bonds .alpha.-1, 4 of the non- reducing ends of homopolysaccharides with at least 5 glucose molecules such as starch, maltodextrin and glycogen, leading to production of glucose-1-phosphate. The GPs in bacteria and animal cells are responsible for the breakdown of glycogen. Although the increase in GP activity leads to a reduction in intracellular levels of glycogen in bacteria and animal cells, this invention discloses the production of plants that have high starch levels and yields and high amylose / amylopectin ratio, as result of the expression of genes coding for GPs.

L'invention concerne un procédé de production de plantes transgéniques qui présentent une teneur et un rendement en amidon élevés et un équilibre amylose/amylopectine élevé. Les alpha-1-4-glucane phosphorylases (GP) catalysent la coupure réversible de liaisons a-1,4 des extrémités non réductrices d'homopolysaccharides à l'aide d'au moins 5 molécules de glucose comme l'amidon, des maltodextrines et le glycogène, donnant lieu à la production de glucose-1-phosphate. Les GP présentes dans des bactéries et des cellules animales sont responsables de la dégradation du glycogène. Même si l'augmentation de l'activité GP entraîne une réduction des niveaux intracellulaires de glycogène dans des bactéries et des cellules animales, l'invention concerne l'obtention de plantes qui possèdent des niveaux et des rendements en amidon élevés, ainsi qu'un équilibre amylase/amylopectine élevé, comme conséquence de l'expression de gènes qui codent pour les GP.

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