A process of aqueous protein extraction from brassicaceae...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/415 (2006.01) A23J 1/14 (2006.01) A23L 1/305 (2006.01) C07K 1/14 (2006.01)

Patent

CA 2688464

A process of aqueous protein extraction from Brassicaceae oilseed meal, such as canola, commercial canola meal or yellow mustard, to obtain a napin-rich protein extract, a cruciferin-rich protein extract, and a low-protein residue. The process comprising the steps of performing aqueous extraction of the Brassicaceae oilseed meal at a pH of from about 2.5 to about 5.0 to obtain a soluble napin-rich protein extract and a cruciferin-rich residue followed by performing aqueous extraction of the cruciferin-rich residue to obtain a soluble cruciferin-rich protein extract and a low- protein residue. The cruciferin-rich residue may be treated with cell wall degrading enzymes to obtain a cruciferin-rich fraction The cruciferin-rich protein products may be substantially free of napin protein and may be useful as a non-allergenic food product for human consumption.

Cette invention concerne un procédé d'extraction aqueuses de protéines contenues dans une farine de graines oléagineuses de Brassicaceae, telle que le canola, la farine de canola ou moutarde blanche, afin d'obtenir un extrait protéique riche en napines, un extrait protéique riche en cruciférines, et un reste à faible teneur en protéines. Le procédé comprend les étapes qui consistent à exécuter l'extraction aqueuse de la farine de graines oléagineuses de Brassicaceae, à un pH compris entre environ 2.5 et environ 5 afin d'obtenir un extrait protéique riche en napines soluble et un reste riche en cruciférines puis à exécuter l'extraction aqueuse du reste riche en cruciférines afin d'obtenir un extrait protéique riche en cruciférines soluble et un reste à faible teneur en protéines. Le reste riche en cruciférines peut être traité au moyen d'enzymes d'altération de parois cellulaires afin d'obtenir un fragment riche en cruciférines. Les produits protéiques riches en cruciférines peuvent être essentiellement exempts de napines et ils peuvent être utilisés en tant que produit alimentaire non allergène comestible pour l'homme.

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