A product removal process for use in a biofermentation system

Details A product removal process for use in a biofermentation system A product removal process for use in a biofermentation system

C - Chemistry, Metallurgy

12

P

C12P 1/00 (2006.01) C12M 1/26 (2006.01) C12N 9/00 (2006.01) D06M 16/00 (2006.01)

Patent

CA 2441774

This invention relates to a bioprocess engineering solution for a product removal process for use in a biofermentation. The invention discloses a process for withdrawing an aliquot of broth from a biofermentation vessel during at least a portion of the biofermentation, removing biocatalyst and water, chromatographically separating biofermentation products from the withdrawn broth using water as an eluent, and returning the remaining components of the broth back to the biofermentation vessel. Process chromatography permits highly selective separation of the target molecule, preventing feedback inhibition of the biofermentation.

La présente invention concerne une solution de génie biologique pour un procédé d'élimination de produit utilisable dans une fermentation biologique. L'invention décrit un procédé permettant le prélèvement d'une aliquote de bouillon à partir d'une cuve de fermentation au moins durant une partie de la fermentation biologique, le prélèvement de biocatalyseur et d'eau, la séparation chromatographique des produits de fermentation biologique à partir du bouillon prélevé utilisant de l'eau en tant qu'éluant, et la restitution des composants restants du bouillon dans la cuve de fermentation biologique. Le traitement chromatographique permet une séparation hautement sélective de la molécule cible, évitant la rétroinhibition de la fermentation biologique.

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