A protease detection product

Details A protease detection product A protease detection product

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01) G01N 33/558 (2006.01)

Patent

CA 2648712

A protease detection product (1) for detecting a protease enzyme in a sample. The protease detection product (1) comprises a medium (2) providing a liquid flow path; a labelled component (6) located on the liquid flow path; a capture component (12) located downstream of the labelled component (6) and immobilisation means (8) for immobilising the intact labelled component. The labelled component (7) comprises a base element (8) connected to releasable element via a protease-sensitive linker peptide (9). The releasable element comprises a label (10). The capture component (12) is capable of binding the releasable element.

La présente invention concerne un produit (1)destiné à la détection d'une enzyme protéase dans un échantillon. Le produit (1) de détection de la protéase est constitué d'un milieu (2) définissant un trajet d'écoulement de liquide, d'un composant marqué (6) localisé sur le trajet d'écoulement de liquide, d'un composant de capture (12) localisé en aval du composant marqué (6) et d'un moyen d'immobilisation (8) destiné à immobiliser le composant marqué intact. Le composant marqué (7) comporte un élément de base (8) relié à un élément libérable par un peptide lieur sensible à la protéase (9). L'élément libérable comporte un marqueur (10). Le composant de capture (12) est capable de se lier à l'élément libérable.

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