A protein fragment complementation assay utilizing e.coli...

C - Chemistry – Metallurgy – 12 – Q

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Details

C12Q 1/00 (2006.01) G01N 33/50 (2006.01) G01N 33/531 (2006.01)

Patent

CA 2411842

The present invention describes an assay method comprising: (A) generating (1) at least a first fragment of a reporter molecule linked to a first interacting domain and at least a second fragment of a reporter molecule linked to a second interacting domain, or (2) nucleic acid molecules that code for (A)(1) and subsequently allowing said nucleic acid molecules to produce their coded products; then, (B) allowing interaction of said domains; and (C) detecting reconstituted reporter molecule activity, where said reporter molecule can react with a penicillin- or a cephalosporin-class substrate.

La présente invention concerne une technique d'essai par laquelle, (A) on commence par générer (1) au moins un premier fragment d'une molécule reporter lié à un premier domaine d'interaction et au moins un deuxième fragment d'une molécule reporter lié à un deuxième domaine d'interaction, ou (2) des molécules d'acide nucléique codant pour (A)(1), puis par laisser ces molécules d'acide nucléique produire leurs produits codés, puis par laquelle (B) on laisse se réaliser les interactions entre lesdits domaines, et enfin par laquelle (C) on détecte l'activité de molécule reporter reconstituée, ladite molécule reporter pouvant réagit avec un substrat de classe pénicilline ou céphalosporine.

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