C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/52 (2006.01) A61K 35/39 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2189646
A purified enzyme mixture useful for isolating cells or cell clusters from tissue is disclosed. The mixture includes at least two collagenase enzymes, at least two other proteases, and additional non-protease components. The mixture is purified by removing at least some of the non-protease components. The purified mixture may then be used to isolate cells or cell clusters from tissue. Also disclosed are the essential components of the purified enzyme mixture as well as preferred ranges and ratios of these essential components for isolating cells or cells clusters from tissue. Finally, a dissociation system is disclosed that can be used with the purified enzyme mixture to dissociate tissue and recover cells or cell clusters.
Mélange d'enzymes purifié utile pour isoler des cellules ou des amas de cellules de tissus, qui comporte au moins deux collagénases, au moins deux autres protéases et des constituants supplémentaires non protéase. Ledit mélange est purifié par élimination d'au moins certains des composants non protéase. Le mélange purifié peut alors être utilisé pour isoler des cellules ou des amas de cellules de tissus. Les constituants essentiels du mélange d'enzymes purifié, ainsi que des gammes et proportions préférées de ces constituants essentiels destinés à isoler des cellules ou amas de cellules de tissus sont également décrits. Enfin, un système de dissociation qui peut être utilisé avec le mélange d'enzymes purifié pour dissocier des tissus et recueillir des cellules ou des amas de cellules est en outre décrit.
Dwulet Francis E.
Ellis Bernice B.
Gill John F.
Jacobsen Linda B.
Smith Marilyn E.
Boehringer Mannheim Corporation
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Roche Diagnostics Operations Inc.
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