A rapid method for microwave mediated enzyme-linked...

G - Physics – 01 – N

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G01N 33/543 (2006.01) G01N 1/44 (2006.01)

Patent

CA 2420011

This invention relates to a rapid and efficient method for carrying out enzyme- linked immunosorbent assay for detection of minute quantities of biomolecules such as antigen, antibody etc. This invention particularly relates to microwave mediated immobilization of antigen or antibody on to the activated surface followed by performing subsequent steps of ELISA by controlled microwave irradiation. The invented procedure has dramatically reduced the total time required for ELISA to less than 10 minutes from hours to days. The invented ELISA procedure is rapid, economical, reproducible and simple and can be automated. The invented procedure is useful for carrying out ELISA in clinical diagnostics, molecular biology, agriculture, sericulture, food technology, environmental science, biomedical research and other related fields.

Cette invention se rapporte à un procédé rapide et efficace de mise en oeuvre d'un dosage immunoenzymatique pour la détection de faibles quantités de biomolécules telles des antigènes, des anticorps, etc. Cette invention se rapporte particulièrement à l'immobilisation, assurée par l'utilisation de micro-ondes, d'un antigène ou d'un anticorps sur la surface activée, puis à la mise en oeuvre des étapes subséquentes de la technique ELISA par irradiation hyperfréquence régulé. Le procédé de cette invention permet de réduire considérablement le temps total requis pour effectuer une ELISA, et de passer ainsi d'une durée de quelques heures, voire quelques jours, à moins de 10 minutes. La technique ELISA mise en oeuvre selon le procédé de l'invention est rapide, économique, reproductible et simple et il est automatisable. Le procédé de cette invention est utile à la mise en oeuvre de la technique ELISA dans les diagnostics cliniques, en biologie moléculaire, en agriculture, en sériciculture, en technique alimentaire, en science de l'environnement, en recherche médicale et autres domaines connexes.

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