A rapid method to detect duplex formation in sequencing by...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) B01J 19/00 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2244120

A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to coalesce into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.

Procédé de détermination de l'existence de doubles hélices de molécules complémentaires d'oligonucléotides, dans lequel on met en contact une pluralité de molécules d'oligonucléotides immobilisés, chacun d'une longueur spécifique et présentant une séquence de nucléotides spécifique, et des molécules d'oligonucléotides simple-brin complémentaires, de façon à former une double hélice pour faciliter l'insertion d'un colorant fluorescent entre les plans des bases de la double hélice. L'invention concerne également un procédé de construction de matrices d'oligonucléotides, consistant à confiner un fluide photochrome sur une surface, à exposer ce fluide photochrome à un motif lumineux de façon à amener le fluide exposé à la lumière à se rassembler en unités discontinues et à adhérer à la surface, et à mettre en contact chacune des unités avec un ensemble de molécules d'oligonucléotides différentes, de façon à permettre aux molécules de se disperser dans les unités.

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