A rapid way to obtain high expression clones of mammalian...

Details A rapid way to obtain high expression clones of mammalian... A rapid way to obtain high expression clones of mammalian...

G - Physics

01

N

G01N 33/53 (2006.01) C12N 1/38 (2006.01) C12N 5/00 (2006.01) G01N 33/567 (2006.01)

Patent

CA 2536675

The invention provides a genetic screening method for identifying a transfected cell expressing the polypeptide of interest. The methods allows for high throughput screening of recombinant cells for elevated levels of expression of the polypeptide of interest. The invention also provides capture media, formulations and methods of making and using thereof.

L'invention concerne un procédé de criblage génétique pour l'identification de cellule transfectée exprimant un polypeptide visé. On assure à travers ce procédé un débit élevé de criblage des cellules de recombinaison, pour des niveaux élevés d'expression du polypeptide visé. L'invention concerne également des milieux de capture, des formulations, et des procédés d'élaboration et d'utilisation correspondants.

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