A recombinant cell line expressing gpcrx11 as a functional...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) A61K 31/7088 (2006.01) A61K 38/17 (2006.01) C07K 14/705 (2006.01) C07K 14/72 (2006.01) C12N 5/10 (2006.01) C12Q 1/68 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2413435

The present invention is related to a G-protein coupled receptor or GPCRx11 similar to rat RTA receptor (37 %) and expressed in testis, thymus and uterus. Aequorin cell line expressing GPCRx11 has been used for screening of tissue extracts and reference ligands. GPCRx11 cells gave a specific signal with synthetic angiopeptin and a somatostatin analog allowing to validate this cell line for screening of natural or synthetic agonists and antagonists. In parallel, extended tissue distribution and polyclonal antibodies have been produced to facilitate GPCRx11 characterisation.

L'invention concerne un récepteur couplé à la protéine G, ou GPCRx11, semblable au récepteur RTA (37%) du rat et exprimé dans les testicules, le thymus et l'utérus. On a utilisé la lignée cellulaire d'aequorine exprimant GPCRx11 pour cribler des extraits tissulaires et des ligands de référence. Les cellules GPCRx11 produisent un signal spécifique avec l'angiopeptine synthétique et un analogue de somatostatine permettant de valider cette lignée cellulaire pour le criblage d'agonistes et antagonistes naturels ou synthétiques. L'invention concerne en outre la production en parallèle d'une répartition tissulaire accrue, et d'anticorps polyclonaux, destinés à faciliter la caractérisation de GPCRx11.

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