C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/56 (2006.01) A23K 1/165 (2006.01) A23L 1/03 (2006.01) A61K 8/66 (2006.01) A61Q 11/00 (2006.01) C12N 1/15 (2006.01) C12N 9/24 (2006.01) C12N 15/62 (2006.01) C12N 15/80 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2258291
The present invention relates to method for constructing an expression vector comprising a mutanase gene obtained from a filamentous fungus suitable for heterologous production comprising the steps of a) isolating a DNA sequence encoding a mutanase from a filamentous fungus, b) introducing a kex2-site or kex2-like site between the DNA sequences encoding the pro-peptide and the mature region of the mutanase, c) cloning the DNA sequence obtained in step b) into a suitable expression vector. The invention also relates to a recombinant expression vector comprising said mutanase gene sequence and a kex2 cleavage site between the DNA sequence encoding the pro-peptide and the region encoding the mature mutanase, a filamentous fungus host cell, a process for producing recombinant mutanase and a recombinant mutanase. It is also the object of the invention to provide compositions useful in oral care products for humans and animals.
Méthode pour produire un vecteur d'expression comprenant un gène de mutanase obtenu à partir d'un champignon filamenteux adapté à la production hétérologue, consistant à a) isoler la séquence d'ADN codant la mutanase dans un champignon filamenteux, b) introduire un site kex2 ou un site de type kex2 entre les séquences d'ADN codant le pro-peptide et la région correspondant à la mutanase mature, c) cloner la séquence d'ADN obtenue à l'étape b) pour former un vecteur d'expression adéquat. L'invention porte également sur un vecteur d'expression recombinant comprenant cette séquence génétique de mutanase et sur un site de coupure kex2 entre la séquence d'ADN codant le pro-peptide et la région codant la mutanase mature, sur une cellule hôte de champignon filamenteux, un procédé pour produire la mutanase recombinante et une mutanase recombinante. L'invention propose en outre des compositions utiles pour les soins buccaux de l'homme et de l'animal.
Fuglsang Claus Crone
Halkier Torben
Hansen Mogens Trier
Johansen Charlotte
Wahleithner Jill Angela
Mccarthy Tetrault Llp
Novo Nordisk A/s
Novozymes A/s
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