A reporter gene

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/56 (2006.01) C12N 9/24 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2470348

Use of a gene which codes a sialidase activity as a reporter gene is described. The gene may be used as a reporter gene in eukaryotic organisms or cells. A modified gene encoding a protein havin g sialidase enzymatic activity is also described. The nucleotide sequence encodes a prokaryotic or eukaryotic sialidase enzymatic activity preferably the nucleotide sequence encodes a Clostridium perfringens sialidase activity.

L'invention concerne l'utilisation d'un gène codant une activité de la sialidase comme un gène rapporteur. Le gène peut être utilisé comme un gène rapporteur dans des organismes ou des cellules eucaryotes. L'invention concerne également un gène modifié codant une protéine possédant une activité enzymatique de la sialidase. La séquence nucléotidique code une activité enzymatique de la sialidase procaryote ou eucaryote, la séquence nucléotidique codant, de préférence, une activité de la sialidase Clostridium perfringens.

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