A robust multi-enzyme preparation for the synthesis of fatty...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/20 (2006.01)

Patent

CA 2706972

Disclosed is an enzymatic process for the preparation of fatty acid alkyl esters, particularly fatty acids methyl esters (biodiesel) in a solvent-free microaqueous system, from a fatty acid source and an alcohol or alcohol donor, employing a robust lipase preparation that comprises at least two lipases separately or jointly immobilized on a suitable support, where one of the lipases has increased affinity to partial glycerides, another is sn-1,3 positional specific, and an optional third lipase has high selectivity towards sn-2 position of the glycerol backbone of the fatty acid source.

L'invention concerne un procédé enzymatique pour la préparation d'esters alkyliques d'acides gras, en particulier d'esters méthyliques d'acides gras (biodiesel) dans un microsystème aqueux exempt de solvants, à partir d'une source d'acides gras et d'un alcool ou d'un donneur d'alcool, utilisant une préparation robuste de lipases qui comprend au moins deux lipases séparément ou conjointement immobilisées sur un support approprié. Une des lipases présente une affinité accrue à des glycérides partiels, une autre est à position spécifique sn-1,3, et une troisième lipase éventuelle présente une sélectivité élevée vis-à-vis de la position sn-2 du squelette du glycérol de la source d'acides gras.

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