A screening method for a substance having hypoglycemic...

G - Physics – 01 – N

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G01N 33/15 (2006.01) C07K 14/47 (2006.01) C12N 15/09 (2006.01) C12Q 1/02 (2006.01) C12Q 1/48 (2006.01) G01N 33/50 (2006.01) G01N 33/66 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2421907

The present invention discloses a screening method for a substance, which could be a therapeutic agent for diabetes with insulin resistance through regulating a function of a molecule involved in insulin signaling pathway. More specifically, the present invention discloses a screening method for a substance having hypoglycemic activity, which is characterized by contacting a sample to STAT-induced inhibitor of STAT function-1 in the presence of insulin, and by detecting inhibitory activity of STAT-induced inhibitor of STAT function-1 by a substance in the sample, as an index.

L'invention porte sur un procédé de criblage de substances utilisables comme remèdes traitant les diabètes insulino-dépendants par régulation de la fonction d'une molécule intervenant dans le mécanisme de transduction des signaux de l'insuline, et plus particulièrement sur un procédé de criblage consistant à mettre le facteur inhibiteur STAT 1 en présence d'insuline et à détecter une substance hypoglycémiante contenue dans le prélèvement en prenant comme indication l'inhibition de l'activité dudit facteur.

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