A screening system for agonists

Details A screening system for agonists A screening system for agonists

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07K 14/47 (2006.01) C07K 14/52 (2006.01) C12N 1/19 (2006.01) C12N 15/81 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2330921

The present invention concerns a screening system for screening effector peptides that can act as agonists or antagonists, as well as means to produce such a screening system, in particular a specific DNA library encoding peptides. In order to perform the screening, host cells comprising a selection system are transformed with the DNA library and the thus obtained screening system is then cultivated under conditions selectively allowing survival of cells with desired interaction.

L'invention concerne un système de criblage qui permet de cribler des peptides effecteurs capables d'agir comme agonistes ou antagonistes, ainsi qu'un mécanisme relatif à l'élaboration d'un tel système, en particulier une librairie d'ADN spécifique pour le codage des peptides. Aux fins du criblage, des cellules hôtes renfermant un système de sélection sont transformées avec la librairie d'ADN, et le système de criblage résultant est mis en culture dans des conditions qui, de manière sélective, autorisent la survie de cellules moyennant l'interaction recherchée.

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