A secretion sequence for the production of a heterologous...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/81 (2006.01) C07K 14/65 (2006.01) C12N 1/19 (2006.01) C12N 9/16 (2006.01) C12N 15/11 (2006.01) C12N 15/18 (2006.01)

Patent

CA 2171557

The invention generally features a novel recombinant DNA molecule useful for producing a heterologous protein, e.g., insulin-like growth factor-I, in yeast. The DNA molecule includes the following sequences joined in frame and in the following 5' to 3' progression: a sequence encoding a signal peptide of a yeast secreted protein; a sequence encoding at least 5 amino acids of the N-terminal region of a mature acid phosphatase protein, a sequence encoding a heterologous protein. Preferably the DNA molecule also encodes a spacer element that includes a proteolytic cleavage site.

L'invention concerne de façon générale une nouvelle molécule d'ADN recombiné utile pour la production dans une levure d'une protéine hétérologue, par exemple le facteur de croissance I insulinoïde. Cette molécule d'ADN comporte les séquences suivantes assemblées en cadre et selon la progression 5' à 3' suivante: une séquence codant un peptide signal d'une protéine secrétée dans une levure, une séquence codant au moins 5 acides aminés de la région terminale N d'une protéine de phosphatase acide de levure mature, et une séquence codant une protéine hétérologue. Cette molécule d'ADN code aussi de préférence un élément d'espacement qui présente un site de clivage protéolytique.

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