C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/54 (2006.01) C07K 1/18 (2006.01) C07K 1/34 (2006.01) C07K 1/36 (2006.01) C12N 15/24 (2006.01)
Patent
CA 2144576
Disclosed is a method for purifying Human interleukin-5 (IL-5) a single chromatographic step. After removing cells from a cell culture expressing human interleukin-5, IL-5 is purified by first adjusting the culture supernatant to the calculated pI value of mature IL- 5 (pI = 7.44) and then passing the conditioned supernatant through tandem linked anion- and cation-exchange columns. The resulting pass- through fraction contains the IL-5 and is devoid of all other contaminating proteins. An optional hydrophobic-interaction chromatography step is disclosed for positive selection of IL-5 and in order to concentrate the preparation. Pure IL-5 was recovered with a high overall yield (>90%), was N-glycosylated and was entirely homodimeric.
Gowling Lafleur Henderson Llp
Merck Frosst Canada & Co./merck Frosst Canada & Cie
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