C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/70 (2006.01) C12Q 1/02 (2006.01) C12Q 1/66 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2267999
Subtle changes in environmental stress can now be detected and measured at sublethal levels as a generic response to environmental stress. The present invention provides an improved method to detect changes in the environmental stress level in very small volumes to permit very high throughput of analyses. The stress change is indicated as a change in the luminescence output of a genetically engineered microorganism. In the present invention, the luminescence gene complex is under the control of a stress inducible promoter. The volumes contemplated range from about 0.5 uL to about 10 mL of media in which the detector organism is suspended.
Il est possible de détecter et mesurer d'infimes variations des contraintes environnementales à des niveaux sublétaux sous forme d'une réponse générique auxdites contraintes. L'invention porte sur un procédé amélioré de détection des susdites variation dans de très faibles volumes, ce qui permet des rendements d'analyse très élevés. Les variations des contraintes sont mises en évidence par des modifications de la luminescence d'un micro-organisme produit par génie génétique. Le complexe de gènes luminescents est commandé par un promoteur réagissant aux contraintes. Les volumes prévus vont d'environ 0,5 uL à environ 10 mL du milieu dans lequel le micro-organisme détecteur est en suspension.
Larossa Robert Alan
Scolnik Pablo Ariel
Bennett Jones Llp
E.i. Du Pont de Nemours And Company
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