A stable hemoglobin reference solution

Details A stable hemoglobin reference solution A stable hemoglobin reference solution

G - Physics

01

N

G01N 33/96 (2006.01) G01N 27/416 (2006.01)

Patent

CA 2049970

A stable reference solution for calibrating and monitoring blood gas instrumentation is disclosed. The solution comprises an aqueous mixture containing a hemoglobin solution derived from a mammalian.source.which comprises at least about 95 % re- duced hemoglobin. The solution additionally contains a bicarbonate buffer and a metal catalyzed methemoglobin reducing sys- tem and an organic buffer. To provide a control element having a variety of properties similar to fresh, whole, human blood, the reference solution is stored in a sealed ampule under an inert atmosphere containing CO2 until just prior to use. The ampule is subsequently opened and the solution is equilibrated with a gas mixture having components which provide gasses in a phy- siological range to the solution. The solution is characterized by exhibiting properties similar to fresh blood subsequent to equilibration and an extended storage life prior to equilibration.

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