C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 11/00 (2006.01) C12P 19/34 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2355816
This invention relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. The method involves single molecule detection of fluorescent labeled PPi moieties released from NTPs as a polymerase extension product is created.
La présente invention concerne un procédé amélioré de séquençage et de génotypage d'acide nucléique dans une configuration à une mono-moléculaire. Le procédé met en oeuvre une détection mono-moléculaire de groupes fonctionnels pyrophosphate (PPi) libérés par les triphosphates nucléotidiques (NTP) pendant la création d'un produit d'extension de polymérase.
Li-Cor Inc.
Ridout & Maybee Llp
LandOfFree
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