A system for stable expression of sirnas in mammalian cells

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) A61K 31/713 (2006.01) A61K 48/00 (2006.01) A61K 49/00 (2006.01) A61P 35/00 (2006.01) C07K 14/47 (2006.01) C12Q 1/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2470903

The present invention provides a polynucleotide comprising a RNA polymerase III promoter, a region encoding a siRNA, and a transcriptional termination element comprising five consecutive thymine residues. The invention also provides for vectors, cells and non-human transgenic animal comprising the polynucleotides of the invention as well as their use in medicaments for various conditions.

La présente invention concerne un polynucléotide contenant un promoteur d'ARN polymérase III, une région codant un ARNsi, et un élément terminal de transcription comportant cinq résidus thymine consécutifs. L'invention concerne également des vecteurs, des cellules et un animal transgénique non humain contenant les polynucléotides selon l'invention, ainsi que l'utilisation desdits polynucléotides dans des médicaments destinés à différents troubles.

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