C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/42 (2006.01) A23K 1/165 (2006.01) C12C 7/04 (2006.01) C12N 15/56 (2006.01) C12N 15/82 (2006.01) C12P 19/14 (2006.01)
Patent
CA 2050468
Novel hybrid thermostable (1,3-1,4)-.beta.-glucanases, their use in food manufacturing and feed manufacturing, DNA frag- ments encoding such glucanases, organisms expressing the DNA fragments and a method for producing the thermostable (1,3 1,4)-.beta.-glucanases. Hybrid fusion genes encoding Bacillus (1,3-1,4)-.beta. -glucanases were constructed, the gene products of which are more thermostable than any (1,3-1,4)-.beta.-glucanase known until now. The hybrid genes were constructed by reciprocal exchanges of the amino-terminal and carboxy-terminal parts of the .beta.-glucanase encoding genes from Bacillus amyloliquefaciens and Bacillus macerans. The resulting thermostable(1,3-1,4)-.beta.-glucanase retain a significant enzymatic activity at temperatures exceeding 65°C and at pH values below 5Ø
Borriss Rainer
Hofemeister Jurgen
Olsen Ole
Thomsen Karl Kristian
von Wettstein Dietrich
Carlsberg A/s
Institut Fur Pflanzengenetik Und Kulturpflanzenforschung
Mccarthy Tetrault Llp
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