A time-domain method and apparatus for determining the depth...

G - Physics – 01 – N

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G01N 21/64 (2006.01) A61B 5/00 (2006.01)

Patent

CA 2544283

Methods and apparatuses for determining the depth and concentration of fluorophores in a turbid medium are disclosed. The method advantageously provides for a rapid estimation of the depth of the flurophore using characteristics of a temporal point spread function. The concentration of the flurophore can be determined using the method of the present invention by combining a calculated depth of the flurophore with a measurement of the intensity of the emitted fluorescence. The intensity can be accurately measured by the apparatus disclosed herein which combine back-reflection and trans-illumination geometries for the source of light injecting and detection.

Méthodes et appareils pour déterminer la profondeur et la concentration d'un fluorophore dans un milieu trouble. La méthode permet d'estimer rapidement et avantageusement la profondeur du fluorophore au moyen des caractéristiques d'une fonction de diffusion en fonction du temps d'arrivée des photons (TPSF). On peut déterminer la concentration en fluorophore en utilisant la méthode décrite en combinant une profondeur calculée du fluorophore avec une mesure de l'intensité de la fluorescence émise. L'intensité peut être mesurée avec précision avec l'appareil décrit, qui associe des géométries de rétroréflexion et de transillumination pour injecter et détecter la source de lumière.

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