C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) C07K 14/435 (2006.01) C12N 15/10 (2006.01) C12N 15/62 (2006.01) C12N 15/90 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2329141
The present application provides a vector for trapping an unknown gene of Drosophila melanogaster, which is a recombinant plasmid comprising the following nucleotide sequences in this order: an artificial consensus splicing acceptor site; a synthetic "stop/start" sequence; a reporter gene; a drug resistance gene; a gene responsible for a detectable phenotype of the Drosophila melanogaster; and a synthetic splicing donor site. The present application also provides a method for trapping an unknown gene of Drosophila melanogaster by using the vector.
L'invention concerne un vecteur servant à piéger un gène inconnu de Drosophila melanogaster, qui consiste en un plasmide de recombinaison comprenant les séquences de nucléotides suivantes dans cet ordre: site accepteur d'épissage de consensus artificiel; séquence synthétique de "marche/arrêt"; gène marqueur; gène de résistance aux médicaments; gène responsable d'un phénotype détectable de Drosophila melanogaster; site donneur d'épissage synthétique. Elle concerne également un procédé servant à piéger un gène inconnu de Drosophila melanogaster au moyen de ce vecteur.
Asztalos Zoltan
Awano Wakae
Lukacsovich Tamas
Yamamoto Daisuke
Japan Science And Technology Corporation
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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