A xylanase obtained from an anaerobic fungus

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/56 (2006.01) A01H 5/00 (2006.01) A23K 1/165 (2006.01) C12N 9/16 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2190194

A xylanase gene, denoted xynC, encoding a novel xylanase (XynC) obtained from strains of the anaerobic fungus Neocallimastix patriciarum is provided. The DNA sequence of the xynC gene is also provided. Transformation of microbial and plant hosts with the xynC gene is described. The xynC gene may be used to design probes for use in hybridization experiments to isolate xylanase genes from other anaerobic fungi. The xynC gene has been used to construct an oleosin-xynC expression construct encoding an oleosin-xylanase fusion protein which retains xylanase activity. Transgenic Brassica napus (canola), transformed with the oleosin-xynC expressionconstruct, expresses the oleosin-xylanase fusion protein which is immobilized in the oil-body membrane of the B. napus seeds. Canola meal, the protein-rich residue left after canola oil is extracted from canola plants, when derived from the transgenic B. napus of the present invention, retains substantial xylanase activity, making it an ideal animal feed supplement.

Un gène de xylanase, noté xynC, codant une nouvelle xylanase (XynC) provenant de souches du champignon anaérobie Neocallimastix patriciarum, est offert. La séquence d'ADN du gène xynC est également offerte. On décrit la transformation d'hôtes d'origine microbienne et végétale par le gène xynC. Le gène xynC peut être utilisé pour concevoir des sondes pour les expériences d'hybridation visant à isoler des gènes de xylanase provenant d'autres champignons anaérobies. Le gène xynC a été utilisé pour élaborer un construit d'expression oléosine-xynC codant une protéine de fusion oléosine-xylanase qui conserve l'activité xylanase. Brassica napus transgénique (canola), transformé à l'aide du construit d'expression oléosine-xynC, exprime la protéine de fusion oléosine-xylanase qui est immobilisée dans la membrane des oléocorps de la graine de B. napus. Lorsqu'elle provient de B. napus transgénique de la présente invention, la farine de canola, le résidu riche en protéine qui reste après extraction de l'huile de canola des plants de canola, conserve une activité xylanase substantielle, ce qui en fait un supplément idéal pour l'alimentation animale.

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