A yeast cell of the genus schwanniomyces

Details A yeast cell of the genus schwanniomyces A yeast cell of the genus schwanniomyces

C - Chemistry, Metallurgy

12

N

195/1.29, 195/1.

C12N 1/19 (2006.01) C12N 15/81 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2015077

The present invention relates to a yeast cell of the genus Schwanniomyces wherein said yeast cell contains at least one expression cassette comprising: a) a first DNA sequence serving as a regulon, b) optionally a second DNA sequence coding for a signal peptide, c) a third DNA sequence coding for a foreign protein, and d) optionally a fourth DNA sequence serving as a terminator. In a preferred embodiment of the invention, the first, second and/or fourth DNA sequences are derived from genes coding for amylolytic enzymes of yeast cells at the genus Schwanniomyces. The use of these functional sequences in an expression cassette provides efficient expression of foreign genes in yeast cells according to the present invention and, furthermore, provides a system for efficient secretion of the expression product into the medium.

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