Abiz phage resistance gene

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/31 (2006.01) A23C 9/123 (2006.01) C07K 14/195 (2006.01) C12N 1/21 (2006.01) C12N 15/74 (2006.01)

Patent

CA 2496630

A first aspect of the present invention is an isolated nucleic acid encoding a phage abortive defense protein, the isolated nucleic acid selected from the group consisting of: (a) isolated nucleic acid having the sequence or coding sequence given in SEQ ID NO: 1 (by "coding sequence" is meant nucleotides 373 to 1668 therein); (b) isolated nucleic acid encoding a phage abortive defense protein and which hybridizes to an nucleic acid having the sequence or coding sequence given in SEQ ID NO: 1, and/or having a sequence at least 60, 70, 80, 90, 92 or 95 percent identical to the sequence or coding sequence given in SEQ ID NO: 1; and (c) isolated nucleic acid encoding a phage abortive defense protein encoded by an isolated nucleic acid of (a) or (b) above, but differing in sequence therefrom due to the degeneracy of the genetic code (e.g., a nucleic acid encoding the protein given in SEQ ID NO: 2).

Selon un premier aspect, l'invention concerne un acide nucléique isolé qui code une protéine de défense abortive bactériophage, cet acide nucléique appartenant au groupe constitué de: (a) acide nucléique isolé à séquence ou séquence de codage propre au numéro de séquence SEQ ID NO: 1 (la "séquence de codage " s'entend des nucléotides 373 à 1668); (b) acide nucléique isolé codant une protéine de défense abortive bactériophage et s'hybridant avec un acide nucléique à séquence ou séquence de codage propre au numéro de séquence SEQ ID NO: 1, et/ou à séquence au moins à 60, 70, 80, 90, 92 ou 95 % identique à la séquence ou à la séquence de codage propre au numéro de séquence SEQ ID NO: 1; et (c) acide nucléique isolé codant une protéine de défense abortive bactériophage codée par un acide nucléique spécifié en (a) ou (b) ci-dessus, mais à séquence différente en raison de la dégénérescence du code génétique (par exemple, acide nucléique codant la protéine prorpe au numéro de séquence SEQ ID NO: 2).

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