Abscription based molecular detection

Details Abscription based molecular detection Abscription based molecular detection

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2755668

The present invention provides methods for detecting biomarkers based on Abscription®, abortive transcription technology. Particularly, the present invention provides bisulfate free methods for detecting methylation of CpG islands from small samples of DNA. The methods are suitable for multiplexing and can be used to analyze multiple CpG islands from a single sample in a short time.

La présente invention concerne des procédés pour détecter des biomarqueurs basés sur Abscription®, la technologie de transcription abortive. En particulier, la présente invention concerne des procédés sans bisulfate pour détecter la méthylation d'îlots CpG à partir de petits échantillons d'ADN. Les procédés sont adaptés pour le multiplexage et peuvent être utilisés pour analyser des îlots CpG multiples à partir d'un échantillon unique en un temps court.

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