Acarbose (acb) cluster from actinoplanes sp. se 50/110

Details Acarbose (acb) cluster from actinoplanes sp. se 50/110 Acarbose (acb) cluster from actinoplanes sp. se 50/110

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) C07K 14/365 (2006.01) C12N 15/74 (2006.01) C12N 15/76 (2006.01) C12P 19/26 (2006.01)

Patent

CA 2282735

The invention concerns biosynthesis genes from the acarbose gene cluster from Actinoplanes sp. SE 50/110, their isolation from Actinoplanes sp. or from producers of pseudooligosaccharides, a process for isolating these biosynthesis genes, the proteins coded by said genes, the expression of the proteins in heterologous host strains, and the use of the acarbose biosynthesis genes for optimizing the process.

L'invention concerne des gènes de biosynthèse issus du groupe de gènes acarbose provenant d'actinoplanes espèce SE 50/110, leur isolement à partir d'actinoplanes d'espèce ou de producteurs de pseudo-oligosaccharides. L'invention concerne un procédé d'isolement de ces gènes de biosynthèse, les protéines codées par lesdits gènes, l'expression des protéines dans des souches-hôtes hétérologues et l'utilisation des gènes de biosynthèse d'acarbose pour optimiser le procédé.

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