Acarbose biosynthesis genes from actinoplanes sp., process...

Details Acarbose biosynthesis genes from actinoplanes sp., process... Acarbose biosynthesis genes from actinoplanes sp., process...

C - Chemistry, Metallurgy

12

N

C12N 15/60 (2006.01) C07K 14/365 (2006.01) C12N 1/21 (2006.01) C12N 9/12 (2006.01) C12N 9/88 (2006.01) C12N 15/52 (2006.01) C12N 15/54 (2006.01) C12P 19/28 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2170577

The invention relates to acarbose biosynthesis genes from actinomycetes, predominantly from Actinoplanes sp. SE 50/110 and its mutants, to a process for the isolation of acarbose biosynthesis genes from actinomycetes using a gene probe which has beenderived from highly conserved protein regions of known dTDP-glucose dehydratase enzymes for finding the genes acbA (coding for dTDP-glucose synthase), acbB (coding for dTDP-glucose dehydratase) and acbC (coding for a cyclase, in part identical to AroB, bacterial 3-dehydroquinate synthases) or one or more acarbose biosynthesisgenes from Actinoplanes sp., and to the use of the acarbose biosynthesis genes.

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