Accelerated nucleic acid reassociation method

Details Accelerated nucleic acid reassociation method Accelerated nucleic acid reassociation method

C - Chemistry, Metallurgy

12

Q

195/1.13, 150/8.

C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 1337753

A method for the formation of double stranded nucleic acid molecules from separate single stranded nucleic acid mole- cules in a single phase reaction solution is disclosed wherein the rate of reaction is greatly increased over the rate of reac- tion at standard reference conditions. The greatly accelerated reaction rate is accomplished through the use of known concentra- tions of nucleic acid precipitating agents which are added to the reaction solution. Nucleic acid denaturing agents may also be added. The solution so formed is incubated and then assayed for the presence of double stranded nucleic acid molecules.

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