Acellular synthesis of cephalosporins

C - Chemistry – Metallurgy – 12 – P

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195/97

C12P 35/00 (2006.01)

Patent

CA 1102264

ABSTRACT OF THE DISCLOSURE A cephalosporin antibiotic is produced from a precursor comprising the five membered thiazolidine ring and the .beta. lactam moiety characteristic of penicillins by contacting the precursor with a cell-free extract of Cephalosporium acremonium in the presence of ATP under conditions favouring high oxygen transfer. Preferably, an ATP regeneration system comprising a phosphate donor and a phosphotransferase enzyme is included in the reaction.

298425

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