Actinomycete promoter

Details Actinomycete promoter Actinomycete promoter

C - Chemistry, Metallurgy

12

N

C12N 15/76 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12N 15/53 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01) C12P 7/62 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2191503

A 1 kbp length of the 5'-noncoding region of the gene encoding cytochrome P-450,sca-2 in the actinomycete Streptomyces carbophilus has transcription promoter activity which is substrate inducible. When the 1 kbp region is shortened, transcription activity becomes constitutive and the shortened promoters can be used advantageously in expression systems, especially those expressing P-450sca-2 in the presence of ML-236B to produce pravastatin sodium.

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