C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/00 (2006.01) C07K 2/00 (2006.01) C07K 14/435 (2006.01) C12N 15/00 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2627413
The present invention relates to a method to produce activated split- polypeptide fragments that on reconstitution immediately forms an active protein. The method relate to real-time protein complementation. Also encompassed in the invention is a method to split and produce split- fluorescent proteins in an active state which produce a fluorescent signal immediately on reconstitution. The present application also provides methods to detect nucleic acids; non-nucleic acid analytes and nucleic acid hybridization in real-time using the novel activated split-polypeptide fragments of the invention.
L'invention concerne un procédé de production de fragments de polypeptides clivés activés lesquels, après reconstitution, forment immédiatement une protéine active. Ce procédé concerne la complémentation protéique en temps réel. L'invention concerne également un procédé de clivage ainsi qu'un procédé permettant de produire des protéines clivées fluorescentes dans un état actif, dans lequel elles produisent un signal fluorescent immédiatement après reconstitution. L'invention concerne encore des procédés de détection d'acides nucléiques, des analytes d'acides non nucléiques ainsi que l'hybridation d'acides nucléiques en temps réel au moyen des nouveaux fragments de polypeptides clivés activés de cette invention.
Broude Natalia
Cantor Charles R.
Demidov Vadim V.
Norton Rose Or S.e.n.c.r.l.,s.r.l./llp
The Trustees Of Boston University
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