Activation of nuclear transfer embryos

Details Activation of nuclear transfer embryos Activation of nuclear transfer embryos

C - Chemistry, Metallurgy

12

N

C12N 5/073 (2010.01) C12N 15/873 (2010.01) A01K 67/027 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2434394

Methods for the activation of nuclear transferred embryos using elevated calcium levels introduced into cells, and a maturation promoting factor (MPF) inhibitor are described. Elevated calcium levels introduced into the embryo cells from a culture medium containing elevated calcium levels, in the range 2 mM to about 12 mM, followed directly and immediately by incubation with DMAP are described. Also described are animals produced from embryos so treated.

L'invention concerne des méthodes d'activation d'embryons de transfert nucléaire, qui mettent en oeuvre des niveaux calciques élevés introduits dans des cellules, et un inhibiteur du facteur promoteur de maturation (MPF). L'invention concerne également l'introduction de niveaux calciques élevés dans des cellules d'embryons à partir d'un milieu de culture contenant des niveaux calciques élevés, de l'ordre de 2 mM à environ 12 mM, suivie directement et immédiatement d'une incubation avec DMAP. L'invention concerne en outre des animaux obtenus à partir d'embryons traités par les méthodes de l'invention.

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