Active retinoic acid free culture medium for avian...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/0735 (2010.01) C12N 5/10 (2006.01)

Patent

CA 2203148

A culture medium for avian totipotential embryonic cells comprising an avian cell culture medium is disclosed. The culture medium is characterised in that it comprises elements complementary to said avian cell culture medium, the complementary elements being selected from the group which comprises cytokines, fibroblast growth factors, insulin-like growth factors and stem cell growth factors, and in that it is substantially free of active retinoic acid. A method for culturing avian totipotential embryonic cells, and the resulting products, are also disclosed.

La présente invention concerne un milieu de culture de cellules embryonnaires totipotentes aviaires du type comportant un milieu de culture pour cellules aviaires, caractérisé en ce qu'il comporte des éléments complémentaires dudit milieu de culture pour cellules aviaires, lesdits éléments complémentaires étant choisis dans le groupe comprenant: les cytokines, les facteurs de croissance des fibroblastes, les facteurs de croissance analogues de l'insuline, les facteurs de croissance des cellules souches, et en ce qu'il est substantiellement dépourvu d'acide rétinoïque actif. Elle concerne également un procédé de culture de cellules embryonnaires totipotentes aviaires et les produits pouvant être obtenus par ce procédé.

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