Active-site titration of glycosyl hydrolases

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/40 (2006.01) C12Q 1/34 (2006.01)

Patent

CA 2536150

The invention provides a method of screening for a property of a glycosyl hydrolase wherein the property is dependent on the concentration of the glycosyl hydrolase. The method comprises a step for determining the concentration of the glycosyl hydrolase by active site titration.

La présente invention a trait à un procédé de criblage pour une propriété d'une glycosyle hydrolase dans laquelle la propriété est dépendante de la concentration de la glycosyle hydrolase. Le procédé comprend une étape pour la détermination de la concentration de la glycosyle hydrolase par la titration des sites actifs.

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