Acyl-coa synthetase

Details Acyl-coa synthetase Acyl-coa synthetase

C - Chemistry, Metallurgy

12

N

195/39, 195/34.8

C12N 3/00 (2006.01) C12N 1/20 (2006.01) C12N 9/00 (2006.01)

Patent

CA 1269943

ABSTRACT OF THE DISCLOSURE An acyl-CoA synthetase is disclosed having the following characteristics: (a) Reaction: Capable of acting on saturated or unsaturated lower to higher fatty acids to produce a CoA derivative thereof (Acyl-CoA), adenosine monophosphate and pyro- phosphoric acid, in the presence of adenosine triphosphate and coenzyme A; (b) Stability: Having a residual activity of not less than about 50% after being incubated in a buffer (pH about 7.5) for about 15 minutes at a temperature of about 55°C; and (c) Optimum reaction temperature: Having an optimum reaction temperature in the range of from 50 to 60°C. Also disclosed is Pseudomonas UKSW-3733 strain which is capable of producing the acyl-CoA synthetase, which has an optimum temperature for growth in the range of from 45 to 55°C and is incapable of assimilating carbonates. The acyl-CoA synthetase is highly stable and, therefore, can be used for preparation of clinical diagnosis kits capable of giving accurate test results over an extended period of time.

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