Acyl-phosphate and phosphonate probes and methods of their...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2521130

The present invention provides tagged acyl phosphate probes ("TAPPs"), and methods of their preparation and use. The subject methods and compositions can provide enhanced simplicity and accuracy in identifying changes in the presence, amount, or activity of target proteins in a complex protein mixture, preferably nucleotide binding proteins using nucleotide binding protein- directed TAPPs. The profiling methods described herein can have a number of steps leading to the identification of target nucleotide binding protein(s) in a complex protein mixture.

La présente invention concerne des sondes d'acyle phosphate marquées (<= TAPP >=) et des procédés pour les synthétiser et les utiliser. Les procédés et les compositions selon cette invention permettent d'identifier plus simplement et plus précisément des variations concernant la présence, la quantité ou l'activité de protéines cibles dans un mélange de protéines complexe, de préférence des protéines de liaison nucléotidique, au moyen de TAPP orientées protéines de liaison nucléotidique. Les procédés de profilage selon cette invention présentent un certain nombres d'étapes menant à l'identification d'une/de protéine(s) de liaison nucléotidique cible(s) dans un mélange de protéines complexe.

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