Adaptation of cell lines to suspension culture

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) C12N 1/00 (2006.01)

Patent

CA 1093994

C-11-21-0279 APPLICATION FOR LETTERS PATENT FOR ADAPTATION OF CELL LINES TO SUSPENSION CULTURE Abstract of the Disclosure Densely packed cells growing rapidly in substrate- attached culture are gently released from the substrate by enzymatic treatment to form aggregates ranging from 20 µ to 500 µ in diameter. These aggregates are then transferred to fresh medium in agitated liquid suspension. The cells thereby continue to grow in aggregates whereby a normally anchorage-dependent established mammalian cell line is rapidly adapted to suspension culture.

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